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Proteins, Peptides and Amino acids
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Pesticide Residue, Endocrine Disruptors and Environmental Poll
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Anions and Cations
  • Guidelines for Shodex Column Selection
    • Non-suppressed Ion Chromatography
    • Suppressed Ion Chromatography
  • Anions(Non-suppressor Method: I-524A Column)
    • Anion Standards (1) (I-524A)
    • Anion Standards (2) (I-524A)
    • Anion Standards (3) (I-524A)
    • Anion Standards (6) (I-524A)
    • Anion Standards (7) (Anions and Organic Acids)
    • Anion Standards (8) (Eluent : Octanesulfonic Acid and Boric Acid)
    • Anion Standards (9) (Polyphosphoric Acids)
    • Effect of pH on Retention Time (1) (I-524A)
    • Dynamic Range of Column
    • Effect of Sample Load on Column Efficiency (1) (I-524A)
    • Tap Water (3) (I-524A)
    • Tap Water (4) (I-524A)
    • River Water (1) (I-524A)
    • Carbonate and Other Anions (1) (I-524A)
    • Sulfate and Sulfite
    • Polythionic Acids
    • Anions in Beer
    • Chinese Cabbage
    • Iodic Ion in Urine
    • Purifed Water
    • Milk
    • Sea Water (1) (I-524A)
    • Sodium Azide (1) (I-524A)
    • Lithium Tetrafluoroborate (I-524A)
  • Anions(Non-suppressor Method: NI-424 Column)
    • Anion Standards (15) (Comparison of NI-424 and I-524A)
    • Anion Standards (16) (NI-424: Phthalic Acid aq. Eluent)
    • Anion Standards (17) (Hydrophobic Inorganic Ions)
    • Anion Standards (18) (NI-424)
    • Tap Water (7) (NI-424)
    • River Water (3) (NI-424)
    • Rain Water
    • Effect of pH on Retention Time (NI-424)
    • Effect of Sample Load on Column Efficiency (4) (NI-424)
    • Linearity of Calibration Curves
    • Calibration Curves for Low Concentration F -
    • Carbonate and Other Anions (2) (NI-424)
    • Phosphonates
    • Analysis of Pyrophosphate and Tripolyphosphate
    • Anion Standards (21)(NI-424 Organic acid)
  • Anions(Non-suppressor Method: Other Columns)
    • Organic Acids
    • Sulfite in Wine (1) (KC-811)
    • Silicic Acid and Boric Acid
    • Sulfite in Wine (3) (KC-811)
    • Arsenic Acid and Arsenious Acid
    • Sulfide Ion and Cyanide Ion (DEAE-825)
    • Simultaneous Analysis of Carbonic ion and Seven Common Anions
    • Effect of Pretreatment of Mineral Water (SI-90 4E)
  • Anions(Suppressor Method: SI-90, SI-50, SI-52 Columns)
    • Anion Standards (19) (SI-90 4E)
    • Anion Standards (20) Simultaneous Analysis of HS (-), SO3 (2-), SO4 (2-) and S2O3 (2-)
    • Rapid Analysis of Anions
    • Mineral Water (2) (SI-90 4E)
    • Low Concentration Anion Standards (3) (SI-90 4E)
    • Effect of Temperature on Elution Time
    • Effect of Na 2 CO 3 Concentartion on Elution Time
    • Effect of NaHCO 3 Concentartion on Elution Time
    • Effect of Flow Rate on Column Efficiency
    • Anion Standards and organic acids (2) (SI-90 4E)
    • Separation of Phosphate, Phosphtite and Hypophosphite
    • Sulfite in Wine (2) (SI-90 4E)
    • Anion Standards (21) (Methane Sulfonic Acid)
    • Hydrophobic Anions (SI-90 4E)
    • Sodium Azide (2) (SI-90 4E)
    • Anion Standards and Organic Acids (3) (SI-50 4E)
    • Bromate in Drinking Water
    • Analysis of Oxyhalides (SI-52 4E)
    • Calibration Curves for Oxyhalides (SI-52 4E)
    • Mineral Water (3) (by Post-column Method)
    • Tap Water (8) (by Post-column Method)
    • Anion Standards (22) (SI-91 4C)
    • Effects of added acetonitrile on the retention time (SI-90 4E)
    • Analysis of organic acids and oxyhalides (SI-52 4E)
    • Analysis of oxyhalides and inorganic ions (SI-52 4E)
    • Sulfamic acid (SI-90 4E)
  • Cations(Y-521)
    • Alkali Metal Ions
    • Alkaline Earth Metal Ions
    • Transition Metal Ions (1) (Y-521)
    • Alkylamines (1) (Y-521)
    • Tap Water and Rain Water
    • Tap Water and Red Wine
    • Selective Detection of Alkaline Earth Ions with Addition of EDTA
  • Cations(Simultaneous separation of monovalent and divalent cations: YS-50, YK-421)
    • Monovalent and Divalent Cation Standards (6) (Comparison of YS-50 and YK-421)
    • Linearity of Calibration Curves (YS-50)
    • Separation of Na + and NH 4 + (1) (Non-suppressor Method)
    • Effect of Methanesulfonic Acid Concentration on Elution Time (YS-50)
    • Effect of Flow Rate on Plate Number (YS-50)
    • Effect of Sample Load on Plate Number (YS-50)
    • Monovalent and Divalent Cation Standards (7) (Eluent Containing Acetonitrile)
    • Eluent to be Used for YS-50
    • Separation of Na + and NH 4 + (2) (Suppressor Method)
    • Cations and Alkylamines (YS-50)
    • Mineral Water and Tap Water (YS-50)
    • Sea Water (2) (YS-50)
    • Red Wine (3) (YS-50)
    • Simultaneous Analysis of Monovalent and Divalent Cations
    • Monovalent and Divalent Cation Standards (1)
    • Monovalent and Divalent Cation Standards (2)
    • Tap Water (1) (YK-421)
    • Dynamic Range of Column
    • High Sensitive Detection of Cations
    • Ethanolamines and Alkylamines
    • Red Wine (1) (YK-421)
    • Cations in Beer
    • Monovalent and Divalent Cation Standards (3) (Dipicolinic Acid + Crown Ether Eluent)
    • Serum
    • Effect of Acetonitorile Concentration on Elution Volume
    • Long Chain Alkylamines
    • Monovalent and Divalent Cation Standards (4) (Phosphoric Acid Eluent)
    • Analysis of Tap Water (2) (YK-412) (Phsphoric Acid Eluent)
    • Monovalent and Divalent Cation Standards (5) (Phosphoric Acid +
    • Alkylamines (2) (YK-421) (Phosphoric Acid + Acetonitrile)
    • Cation Analysis in the Presence of PEG (YS-50)
    • Cation Analysis in the Presence of Albumin (YS-50)
    • Monovalent and Divalent Cation Standards (8) (Ethylenediamine Containing Sample)
    • Monovalent and Divalent Cation Analysis in River Water (Ethylenediamine Containing Sample)
    • Monovalent and Divalent Cation Analysis in Tap Water (Ethylenediamine Containing Sample)
  • Transition Metal Ions and Rare Earth Metal Ions
    • Transition Metal Ions (2) (T-521)
    • Transition Metal Ions (3) (YS-50)
    • Red Wine (2) (T-521)
    • Tap Water (2)
    • Cobalt Ion by Chemiluminescence Detection
    • Rare Earth Metal Ions
    • Yttrium Ore
    • Nd-Dy Magnetic Alloy
    Column Switching
    Other Applications
    NP & RP (Normal & Reversed Phase)
    SEC (Size Exclusion Chromatography)
    Saccharides & Organic Acids Analysis
    IEX (Ion Exchange Chromatography)
    IC (Ion Chromatography)
    HIC (Hydrophobic Interaction Chromatography)
    Affinity Chromatography
    Chiral Separation
    LC/MS & Rapid Analyses of Medicines
    Exclusive Use
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    Monovalent and Divalent Cation Standards (3) (Dipicolinic Acid + Crown Ether Eluent)

    The problems in the anaysis using IC YK-421 is that the analysis of very small amount of NH4+ in a sample which contains large amount of Na+ is difficult. Addition of 1.5mM 18-Crown 6-ether in 5mM Tartaric acid + 1mM Dipicolinic acid + 1.5g/L Boric acid eluent can solve the problem. Addition of crown 6-ether can adjust the elution volume of the cations. Especially, the elution volume of K+ is largely affected by the additon and elute after Mg2+.


    Sample :
    1. Li+
    2. Na+
    3. NH4+
    4. Ca2+
    5. Mg2+
    6. K+

    Column       : Shodex IC YK-421 (4.6mmID*125mm)
Eluent       : 5mM Tartaric acid + 1mM Dipicolinic acid 
               + 1.5g/L Boric acid + 1.5mM 18-Crown 6-ether aq.
Flow rate    : 1.0mL/min
Detector     : Shodex CD
Column temp. : 40°C

     
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