Amino Acids (3) (P-421S)
Amino acid standards are analyzed using CXpak P-421S. The column pressure is low (3MPa under 0.5mL/min and 63°C), because of the 6µm of gel is packed into this column. We show the chromatogram of standard amino acids under ninhydrine reaction. 17 kinds of amino acids are separated by baseline. The total time for analysis is within 50 minutes till the last peak of arginine.
Sample :
1. Aspartic acid, Asp
2. Threonine, Thr
3. Serine, Ser
4. Glutamic acid, Glu
5. Proline, Pro
6. Glycine, Gly
7. Alanine, Ala
8. Cysteine, Cys
9. Valine, Val
10. Methionine, Met
11. Isoleucine, Ile
12. Leucine, Leu
13. Tyrosine, Tyr
14. Phenylalanine, Phe
15. Lysine, Lys
16. NH3
17. Histidine, His
18. Arginine, Arg
Column : Shodex CXpak P-421S (4.6mmID*150mm)
Eluent : No.1; 0.12M Sodium citrate buffer(pH3.3)
No.2; 0.13M Sodium citrate buffer(pH3.2)
No.3; 0.11M Sodium citrate buffer(pH4.0)
No.4; 1.02M Sodium citrate buffer(pH4.9)
No.5; 0.2M NaOH + 10% C2H5OH (Rinse solution)
Low pressure gradient:
0min; No.1, 1.2min; No.2, 10.0min; No.3, 21.2min; No.4, 40.0min; No.5
Flow rate : 0.5mL/min
Detector : VIS(570nm) (Ninhydrine reaction; 0.35mL/min, 120°C)
Column Temp. : 63°C