Amino Acids (5)

Amino acids were analyzed using ion (cation) chromatography column, YS-50. The base material used for YS-50 is polyvinyl alcohol, modified with carboxyl functional group. It works under reverse-phase mode and ionic interactions to separate amino acids, which is similar to the separation mode used in NN-814 column.


Sample : 5µL
1. Aspartic acid 0.8mg/mL
2. Glutamic acid 0.8mg/mL
3. Glycine 0.8mg/mL
4. Valine 1.0mg/mL
5. Methionine 1.0mg/mL
6. Isoleucine 1.0mg/mL
7. GABA 1.0mg/mL
8. Phenylalanine 1.0mg/mL
9. Ornithine 1.0mg/mL
10. Histidine 1.0mg/mL
11. 1-Methylhistidine 1.0mg/mL
12. Arginine 1.0mg/mL

Column       : Shodex IC YS-50 (4.6mmID*125mm)


Eluent       : 6mM H3PO4 aq.


Flow rate    : 1.0mL/min


Detector     : Shodex RI


Column temp. : 40° C