Features of Multimode Columns (Peptide Analysis)
Although Asahipak GS-HQ series is basically GFC columns, they permit different types of separation and analysis from conventional ones by use of a multimode which combines the hydrophobic interaction (RPC) and ionic interaction (IEX: ion exclusion). The GS-320 HQ column performs well in separating hydrophillic peptides, particularly acidic and basic peptides. As the columns can be used over a wide range of eluent pH because of being polymer-based column, they can be washed with alkaline solution leading to a longer column life, which is another feature of the column.
1. Val-Glu-Glu-Ala-Glu, 2. Glu-Ala-Glu, 3. Val-Glu-Ser-Glu
4. Arg-Asp, 5. Gly-His-Lys, 6. Arg-Pro-Lys-Pro
Column : Shodex Asahipak GS-320 HQ (7.6mmID*300mm) Eluent : 50mM Ammonium acetate buffer(pH6.7) Flow rate : 0.5mL/min Detector : UV(220nm) Column temp. : 30°C