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Proteins, Peptides and Amino acids
Nucleic Acids
Saccharides and Organic Acids
  • Guidelines for Shodex Column Selection: Saccharides and Organic acids
    • Column Selection: by Elution
    • Explanation on Ligand Exchange Mode
    • Column Selection
  • Monosaccharides and Oligosaccharides
    • Elution Volume of Saccharides
    • Separation of Anomers
    • Comparison of Amino Column with Amide Column
    • Comparison of Columns with Different Counter Ions
    • Combination of Columns with Different Counter Ions
    • Elution Volume of Saccharides and Sugar Alcohols
    • Effect of Free BaseRatio of Amino Group on Plate Number
    • Effect of Acetonitrile Concentration on Elution Time (NH2P-50)
    • Effect of Acetonitrile Concentration on Elution Time (SZ5532)
    • Effect of Acetonitrile Concentration on Plate Number
    • Durability against Acidic Solvents
    • Durability against Alkaline Solvents
    • Durability against Eluent Composition Change
    • Effect of Polysaccharide Adsorption
    • Effect of Sample Solvent Composition
    • Effect of Sample Injection Volume
    • Effect of Flow Rate (SC1011)
    • Effect of Temperature (1)
    • Effect of Temperature (2)
    • Calibration Curves for Saccharides (NH2P-50)
    • Calibration Curves for Saccharides (SZ5532)
    • Comparison of NH2P with Silica-based Amino Column (1)
    • Comparison of NH2P with Silica-based Amino Column (2)
    • Monosaccharides (1)
    • Monosaccharides (2)
    • Monosaccharides (3)
    • Monosaccharides and Disaccharides (1)
    • Monosaccharides and Disaccharides (2)
    • Monosaccharides and Disaccharides (3)
    • Mono-, Di- and Trisaccharides (1)
    • Mono-, Di- and Trisaccharides (2)
    • Saccharides in Agricultural Products
    • Saccharides in Wood
    • Palatinose in Food
    • Worcester Sauce
    • Yogurt: Sugar added (SP0810)
    • Yogurt: Sugar added (NH2P-50)
    • Sweet Potato, roasted (NH2P-50)
    • Sweet Potato, roasted (SP0810)
    • Soybean Flour
    • Milk Coffee
    • Jelly
    • Bread
    • Raspberry Jam
    • Candy
    • Lactic acid Beverage and Fermented Milk
    • Orange and Melon
    • Orange Juice
    • Chocolate Cake (NH2P-50)
    • Chocolate Cake (SZ5532)
    • Maltose and Isomaltose
    • Saccharide Analysis Using Semi-micro Column
    • Saccharides in Powdered Milk
    • Glucose Derivatives
    • Extract of Wheat Rod
    • Effect of Organic Solvent in Eluent
    • Saccharides in Food
    • Analysis of Soft Drinks (LC/MS)
    • Monosaccharides Composing Sugar Chain
    • Maltose and Nigerose
    • Sugar Separation in Presence of Na salt (DC-613)
    • Meglumine (NN-414)
    • Heptulose Separation (KS-801)
  • Oligosaccharides
    • Hydrolyzed Dextran (1)
    • Hydrolyzed Dextran (2)
    • Hydrolyzed Dextran (3)
    • Isomalto-oligosaccharides
    • Hydrolyzed Dextran (5)
    • Maltooligosaccharides
    • N-Acetyl-Chitooligosaccharides
    • Chitosan-oligosaccharides (Chitooligosaccharides) (1)
    • Chitosan-oligosaccharides (Chitooligosaccharides) (2)
    • Cyclodextrins
    • Oligosaccharides and Sugar Alcohols
    • Short-Chain Amylose (1)
    • Short-Chain Amylose (2)
    • Hydrolyzed Starch (2)
    • Sweetener
    • Effect of Flow Rate (SC1211)
    • Fructooligosaccharide Syrup
    • Starch Syrup
    • Dietary Fiber
    • Analysis of Flucto-oligo Sugar Components (KS-802+KS-801)
  • Acid Saccharides, Phosphorylated Saccharides
    • Oligogalacturonic Acids
    • Phosphorylated Saccharides (1)
    • Phosphorylated Saccharides (2)
    • Phytic acid
  • Saccharides
    • Separation of N-acetylneuraminic Acid and N-glycolyneuraminic Acid
  • Saccharides, Sugar Alcohols, Aminosugars and Amino Acids.
    • Sugar Alcohols (1) (SC1211)
    • Sugar Alcohols (2) (NH2P-50)
    • Sugar Alcohols (3) (KS-801)
    • Effect of Acetonitrile Concentration
    • Saccharides and Sugar Alcohols (1) (SZ5532)
    • Saccharides and Sugar Alcohols (2) (SP0810)
    • Saccharides and Sugar Alcohols (3) (SP0810)
    • Saccharides and Sugar Alcohols (4) (SC1011)
    • Saccharides and Sugar Alcohols (5) (SZ5532)
    • Saccharides and Sugar Alcohols (6) (NH2P-50)
    • Saccharides and Sugar Alcohols (7) NH2P, LC/MS
    • Amino Sugars
    • Saccharides and Amino Sugars (1)
    • Saccharides and Amino Sugars (2)
    • Saccharides and Amino Acids (1)
    • Saccharides and Amino Acids (2)
    • Mannitol
    • Palatinit (Isomalt)
    • Xilitol in Confectionery
    • Soft Drink Containing Erythritol
    • Apple Juice (NH2P-50)
    • Apple Juice (SC1011)
    • Sake (Japanese Rice Wine)
    • The comparison between NH2P-40 3E and its conventional type (NH2P-50 4E)
  • Saccharides and Organic Acids
    • Elution Volume of Saccharides and Organic Acids
    • Saccharides and Organic Acids (1)
    • Saccharides and Organic Acids (2)
    • Lactic acid and Acrylic acid
    • Calibration Curve for Saccharides and Organic Acids (SH1011)
    • Soft Drink
    • Analysis of Saccharides and Organic Acids (RI and UV Detector)
    • Fermentation By-products (Model Solution)
  • Saccharides and Sweetning agents(Additives for Foods)
    • Preservatives (1) (DS-613)
    • Preservatives (2) (DE-613)
    • Preservatives (4) (DE-413)
    • Acesulfame K
    • Sucralose
    • Food Yellow No.4 (Tartrazine)
    • Effect of Eluent pH on Analysis Result
    • Benzoates
    • Alkylbenzenes (1) (DS-613)
    • Alkylbenzenes (2) (DS-613)
    • Alkylbenzenes (4) (DE-413)
    • Polycyclic Aromatic Hydrocarbons
    • Phenols (2)
    • Chlorobenzenes
    • Iodobenzene Diacetate
    • Anilines
    • Perylene
    • Durability of ODP Column with Alkaline Eluent
    • Phenylenediamines
  • Polysaccharides
    • Calibration Curves for KS-800 Series
    • Calibration Curves for KS-800 Series (Column Combination)
    • Amylose
    • Carboxymethylcellulose (1)
    • Carboxymethylcellulose (2)
    • Hyaluronic Acid (1) (SB-805 HQ)
    • Hyaluronic Acid (2) (SB-806M HQ)
    • Hyaluronic Acid (3) (KS-807 vs SB-806 HQ)
    • Hyaluronic Acid (4) (SB-807 HQ)
    • Corn Starch (1) (Aqueous Eluent)
    • Corn Starch (2) (DMSO/DMF Eluent)
    • Chondroitin Sulfate A (KS-804)
    • Chondroitin Sulfate Na
    • Chondroitin Sulfate (SB-806M HQ)
    • Chitosan
    • Hydroxyethyl cellulose(1) SB-806M HQ
    • Pullulan and Oligosaccharides
    • Pullulan, Saccharides and Ethanol
    • Heparin (1) (SB-804 HQ)
    • Heparin (2) (SB-806M HQ)
    • Glycogen (1) (KS-806)
    • Glycogen (2) (SB-806M HQ)
    • Preparation of CS-Gulcan in Coix Seed
    • Inulin (1) (KS-806)
    • Inulin (2) (SB-806M HQ)
    • Mucin (SB-806M HQ)
    • Pectin (SB-806M HQ)
    • Sodium Alginate (SB-806M HQ)
    • Carrageenan (SB-806M HQ)
    • Keratan Sulfate (SB-806M HQ)
    • Hydrolyzed Starch (1)
  • High-sensitive, High-selective Analysis of Saccharides
    • Cyclodextrin in Plasma
    • Glucose
    • N-Acetylglucosamine and N-Acetylchitobiose
    • p-Nitrophenyl-galactoside, -glucoside and -mannoside
    • p-Nitrophenyl-glucopyranoside and -galactopyranoside
    • Mannose, Mannitol and Sorbitol
    • Pyridylamino Sugar Chain Analysis
  • Organic Acids
    • Effect of Flow Rate (RSpak KC-811)
    • Effect of Injection Volume (RSpak KC-811)
    • Effect of Sample Load (RSpak KC-811)
    • Effect of Column Temperature (RSpak KC-811)
    • Effect of Concentration of Eluent on Elution Volume
    • Effect of Column Temperature on Elution Volume (1)
    • Effect of Column Temperature on Elution Volume (2)
    • Effect of Acetonitrile in Eluent (1)
    • Effect of Acetonitrile in Eluent (2)
    • Effect of Reversed Phase Column on Elution Volume
    • Elution Volume of Aromatic Organic Acids
    • Elution Volume of Organic Acids (RSpak NN-814)
    • Elution Volume of Organic Acids (RSpak DE-413)
    • Comparison of Analysis of Organic Acids with 4 Kinds of Detectors(Standards)
    • Comparison of Analysis of Organic Acids with 4 Kinds of Detectors(Soy sauce)
    • Organic Acid Standards (1)
    • Organic Acid Standards (2)
    • Organic Acid Standards (3)
    • Organic Acid Standards (4) (DE-413)
    • Organic Acid Standards (5) (DE-213)
    • Di-carboxylic Acids
    • Analysis of Organic Acids (Eluent Conteinig Cyclodextrin)
    • Succinic Acid and Fumaric Acid
    • Citric Acid in Phosphoric Acid
    • Organic Acids in Phosphoric Acid
    • Organic Acids in Beer
    • Organic Acids in Soy Source
    • Organic Acids in Vinegar
    • Organic Acids and Vitamin C in Fruit Juice
    • Gluconic Acids
    • Sake (Japanese Rice Wine)
    • Organic Acids in White Wine
    • Oxalic Acid
    • Organic Acids in Liquid for Permanent Wave
    • Camphor and Benzoic Acid
    • Organic Acids in Soybean Paste
    • Organic Acids in Soy Sauce For Noodles
    • Cellooligosaccharides and Furfurals (SH1821)
    • Organic Acids analysis in Black-vinegar (KC-811)
  • Miscelleanous
    • Furfural and Guaiacol
    Drugs, Metabolites and Chiral Compounds
    Vitamins, Hormones and Lipids
    Pesticide Residue, Endocrine Disruptors and Environmental Poll
    Synthetic Polymers
    Anions and Cations
    Column Switching
    Other Applications
    NP & RP (Normal & Reversed Phase)
    SEC (Size Exclusion Chromatography)
    Saccharides & Organic Acids Analysis
    IEX (Ion Exchange Chromatography)
    IC (Ion Chromatography)
    HIC (Hydrophobic Interaction Chromatography)
    Affinity Chromatography
    Chiral Separation
    LC/MS & Rapid Analyses of Medicines
    Exclusive Use
    Calibration Standards for SEC
    Column Switching
    GPC Clean-up
    Detectors
    New products
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    Sake (Japanese Rice Wine)

    Three kinds of Japanese sake (wine), sweet, medium and dry, were analyzed using SUGAR SC1211 (a column for saccharides analysis). It was confirmed that the sweet sake had the largest sugar content.


    Sample : Sake
    1. Maltotriose
    2. Maltose
    3. Glucose
    4. Glycerol
    5. Ethanol

    Column       : Shodex SUGAR SC1211 (6.0mmID*250mm)
    Eluent       : H2O 
    Flow rate    : 1.0mL/min
    Detector     : Shodex RI
    Column temp. : 80°C

     
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