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Proteins, Peptides and Amino acids
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Pesticide Residue, Endocrine Disruptors and Environmental Poll
Synthetic Polymers
  • Basic Information on GPC
    • To Realize Pure GPC Mode
    • Solubilities of Solvents
    • Physical Chemistry Properties of Solvents
    • Polarities of Solvents
  • Guidelines for Shodex Column Selection: Synthetic Polymers
    • Hydrophobicity of Columns
  • Calibration Curves
    • Calibration Curves for KF-800 Series
    • Effect of Solvent-Peak Separation Column on Calibration Curve
    • Calibration Curves for KF-600 Series
    • Calibration Curves for KF-400HQ Series (1) (Low Molecular Weight)
    • Calibration Curves for KF-400HQ Series (2) (High Molecular Weight)
    • Calibration Curves for KF-806L (Various Eluents: PS)
    • Comparison of Caliburation Curves for LF-404 and LF-604
    • Calibration Curve for LF-604 (DMF Eluent: PEG, PEO)
    • Calibration Curve for LF-604 (NMP Eluent: PEG, PEO)
    • Calibration Curve for LF-604 (NMP Eluent: PS)
    • Calibration Curves for KD-800 Series
    • Calibration Curves for HFIP-800 Series (HFIP Eluent: PMMA)
    • Calibration Curves for HFIP-600 Series (HFIP Eluent: PMMA)
    • Calibration Curves for KW-800 Series
    • Calibration Curves for KW-802.5 (Proteins , Pullulan, PEG and P
    • Calibration Curves for KW-803 (Proteins , Pullulan, PEG and PEO)
    • Calibration Curves for KW-804 (Proteins , Pullulan, PEG and PEO)
    • Calibration Curves for SB-800 HQ Series (Aqueous Eluent: Pullulan)
    • Calibration Curves for SB-800 HQ Series (Aqueous Eluent: PEO, PEG)
    • Calibration Curves for SB-800 HQ Series (DMF Eluent: PS)
    • Calibration Curves for SB-800 HQ Series (DMF Eluent: PEG, PEO)
    • Calibration Curves for SB-800 HQ Series (DMF Eluent: PMMA)
    • Calibration Curves for SB-806M HQ (Aqueous Eluent: Pullulan, PEG and PEO)
    • Calibration Curves for SB-806M HQ (DMF Eluent: PEG, PEO and PS)
    • Comparison of Calibration Curves for KD-800 Series and SB-800 HQ Series (DMF Eluent)
    • Comparison of Calibration Curves for KD-802.5 and SB-802.5 HQ (DMF Eluent: PEG, PEO and PS)
    • Calibration Curves for GS-HQ Series (Aqueous Eluent: Pullulan and PEG)
    • Calibration Curves for GF-HQ Series (Aqueous Eluent: Proteins)
    • Calibration Curves for GF-HQ Series (Chloroform Eluent: PS)
    • Calibration Curves for GF-HQ Series (Aqueous Eluent: Pullulan Pullulan und PEG)
    • Calibration Curves for GF-HQ Series (DMF Eluent: PS)
    • Calibration Curves for GF-HQ Series (DMF Eluent: PEG, PEO)
    • Calibration Curves for GF-HQ Series (DMF Eluent: PMMA)
    • Calibration Curve for GF-510 HQ + GF-310 HQ (THF Eluent: PS)
    • Calibration Curve for GF-510 HQ + GF-310 HQ (DMF Eluent: PS)
    • Calibration Curve for GF-710 HQ (Methanol Eluent: PEG and PEO)
    • Calibration Curves for UT-800 Series
    • Calibration Curves for HT-800 Series
    • Calibration Curves for HT-806M and AT-806M
    • Calibration Curves of Hydrophilic Polymers (1) (Sodium Poly(Styrene Sulfonate))
    • Calibration Curves of Hydrophilic Polymers (2) (Sodium Polyacrylate)
    • Calibration Curves of Hydrophilic Polymers (3) (Poly(Sodium Methacrylate))
    • Comparison of Calibration Curves for Down-sized GPC Columns (HFIP Eluent: PMMA)
    • Calibration curves for organic SEC
    • Calibration Curve for K-800 series
  • Column Connection and Linear Type Columns
    • Column Connection and Linear Type Columns
    • Calibration Curves for KF-800L Series
    • Calibration Curves for K-800L Series
    • Comparison of Calibration Curves: Linear Type and Mixed Gel Typ
    • Comparison of Calibration Curves: Linear Type and Column Connection
    • Comparison of Calibration Curves: Linear Type and Conventional Type
    • Comparison of Separation: Linear Type and Column Connection
    • Comparison of Separation: Linear Type and Conventional Type (1)
    • Comparison of Separation: Linear Type and Conventional Type (2)
  • Linear Type Column LF-804
    • Analysis of Synthetic Polymers Using Linear Type Column LF-804
    • Eluent Replacement of GPC LF-804
    • Calibration Curve for LF-804 (THF Eluent: PS)
    • Phenoxy Resin (2) (LF-804)
    • Polycarbonate Resin (3) (LF-804)
    • Poly(Vinyl Butyral) (2) (LF-804)
    • Poly(vinyl Formal) (2) (LF-804)
    • Poly(4-Vinylpyridine) (LF-804)
    • Poly(2-Vvinylpyridine-co-styrene) (LF-804)
    • Comparison of LF-804, LF-404 and LF-604
    • The separation of polystyrene standard using GPC columns
  • Linear Type Columns LF-404, 604
    • The Effect of The Unit of Columns on The Separation (1) (LF-404: PS)
    • The Effect of The Unit of Columns on The Separation (2) (LF-404: Epoxy resin)
    • Styrene Butyl Methacrylate Copolymer (2) (LF-404)
    • Poly(Methyl Methacrylate) (LF-404)
    • Ethylene Vinyl Acetate Copolymer (3) (LF-404)
    • 1,2-Polybutadiene (2) (LF-404)
    • Phenoxy Resin (3) (LF-604)
    • Poly(Vinyl Butyral) (3) (LF-604)
  • Downsized Columns KF-600
    • Comparison of KF-602 and KF-802
    • Effect of Temperature (KF-602)
    • Effect of Flow Rate (KF-602)
    • Effect of Sample Concentration (KF-606M)
    • Effect of Injection Volume (KF-606M)
    • Polystyrene Standards (11) (KF-601)
    • Polystyrene Standards (12) (KF-602)
    • Polystyrene Standards (13) (KF-602.5)
    • Polystyrene Standards (14) (KF-603)
    • Polystyrene Standards (15) (KF-604)
    • Polystyrene Standards (16) (KF-606M)
    • Polystyrene Standards (18) RAPRA, NBS 706
    • Epoxy Resin
    • Poly(Dimethylsiloxane): Toluene Eluent
    • Cellulose Acetate (2) (KF-606M)
    • Poly(Vinylpyrrolidone) (2) (DMF Eluent: KF-806M)
    • Styrene Acrylonitrile Copolymer (2) (KF-606M)
    • Styrene Allylalcohol Copolymer (2) (KF-606M)
    • Vinyl Chloride Vinyl Acetate Copolymer (2) (KF-606M)
    • Polyimide: NMP Eluent
    • Poly(Vinylpyrrolidone) (5) (NMP Eluent)
  • Semi-micro GPC Columns KF-400HQ
    • Comparison of KF-402HQ and KF-802
    • Influence of Instruments for Performance of Semi-micro Columns
    • Comparison of Chromatograms of KF-400HQ Series
    • Poly-styrene oligomer separation (KF-402HQ)
    • Effects of Flow Rate (1) (KF-402HQ)
    • Effects of Flow Rate (2) (KF-402.5HQ+KF-403)
    • Effects of Sample Concentration (KF-402HQ)
    • Effects of Sample Concentration (2) (KF-403HQ + KF-402HQ)
    • Effects of Sample Concentration (3) (KF-406LHQ)
    • Effects of Temperature (KF-402HQ)
    • MW distribution analysis (KF-406L HQ)
  • Solvent-peak Separation Columns
    • Effect of Solvent-peak Separation Column on Separation (1)
    • Effect of Solvent-peak Separation Column on Separation (2)
  • Hydrophobic Polymers: THF Eluent
    • Polystyrene Standards (1) (KF-801)
    • Polystyrene Standards (2) (KF-802)
    • Polystyrene Standards (3) (KF-803L)
    • Polystyrene Standards (4) (KF-804L)
    • Polystyrene Standards (5) (KF-805L)
    • Polystyrene Standards (6) (KF-806L)
    • Polystyrene Standards (7) (KF-807L)
    • Polystyrene Standards (21) (THF, Chloroform and DMF)
    • Poly(Ethylene Glycol) Standards (1) (THF Eluent)
    • Poly(Methyl Methacrylate) Standards (1) (KF-805L)
    • Poly(Methyl Methacrylate) Standards (2) (KF- 806L)
    • Polycarbonate Resin (1) (KF-806L)
    • Phenoxy Resin (1) (KF-806L)
    • Phenol Resin (1) (KF-804L)
    • Polycaprolactone
    • 1,2-Polybutadiene (1) (KF-805L)
    • Poly(Vinyl Formal) (1) (KF-806L)
    • Poly(Vinyl Butyral) (1) (KF-806L)
    • Cellulose Acetate (3) (KF-806L)
    • Cellulose Acetate Butyrate
    • Poly(Vinyl Chloride)
    • Poly(Vinyl Acetate)
    • Ethylene Vinyl Acetate Copolymer (1) (KF-806L)
    • Poly(Butyl Methacrylate)
    • Poly(Isobutyl Methacrylate)
    • Butyl Methacrylate Isobutyl Methacrylate Copolymer
    • Polysiloxane
    • Vinylidene Chloride Vinyl Chloride Copolymer
    • Vinyl Chloride Vinyl Acetate Vinyl Alcohol Terpolymer
    • Styrene butyl methacrylate copolymer (1) (KF-805L)
    • Styrene Butadiene ABA Block Copolymer
    • Styrene Isoprene ABA Block Copolymer
    • Styrene Ethylene Butylene ABA Block Copolymer
    • Poly(2,4,6-tribromostyrene)
    • Polysulfone Resin
    • Polyurethane (LF-404)
  • Hydrophobic Polymers: Chloroform Eluent
    • Polystyrene Standards (8) (K-804L)
    • Polystyrene Standards (17) (K-806M)
    • Epoxy Resin (2) (K-803L)
    • Biodegradable Polymer (1) (Bionolle)
    • Poly(o-Cresyl Glycidyl Ether)-co-Formaldehyde
    • Poly(Ether Ether Ketone)
    • Poly(Butylene Terephthalate) (2) (Eluent: Chloroform)
    • Biodegradable Polymer (2) (Polylactic Acid)
    • Liquid Paraffin
    • Low MW polydimethylsiloxane (chloroform)
  • Hydrophobic Polymers: DMF Eluent
    • Poly(Ethylene Oxide) Standards
    • Poly(Ethylene Glycol) Standards (2) (DMF Eluent)
    • Polystyrene Standards (9) (KD-802)
    • Phenol Resin (2) (KD-803 + KD-802)
    • Phenoxy Resin (2) (KD-806M)
    • Melamine Resin
    • Melamine Formaldehyde Resin (1) (DMF Eluent)
    • Poly(Vinylpyrrolidone) (1) (DMF Eluent: KD-806M)
    • Poly(Vinylpyrrolidone) (4) (DMF Eluent: Effect of LiBr Concentration)
    • Poly(Vinylidene Fluoride)
    • Cellulose Acetate (1) (KD-806M)
    • Cellulose Acetate (4) (DMF Eluent: Effect of LiBr Concentration)
    • Chlorinated Rubber
    • Styrene Allylalcohol Copolymer (1) (KD-803 + KD-802)
    • Styrene Maleic Anhydride Copolymer
    • Styrene Acrylonitrile Copolymer (1) (KD-806M)
    • N-Vinylpyrrolidone Vinyl Acetate Copolymer
    • Vinyl Chloride Vinyl Acetate Copolymer (1) (KD-806M)
    • Vinylidene Chloride Acrylonitrile Copolymer (1) (KD-806M)
    • Poly(-N,N-Dimethyl Aminomethyl Styrene) (1) (DMF Eluent)
    • Ethylene Maleic Anhydride Copolymer
    • Vinylidene Chloride Acrylonitrile Copolymer (2) (Comparison of
    • Phenol Formaldehyde Resin
    • Polycarbonate Resin (2) (Comparison of SB-800 HQ and KD-800)
  • Hydrophobic Polymers: HFIP Eluent
    • Polyamide (1) Nylon6 (HFIP-806M)
    • Polyamide (2) Nylon6/6 (HFIP-805 + HFIP-803)
    • Polyamide (3) Nyon12/2 (HFIP-806M)
    • Polyamide (4) Nylon6/10 and Nylon6 (HFIP-606M)
    • Polyamide (5) Nylon6/6 (LF-604)
    • Polyamide (6) Nylon6 (LF-604)
    • Poly(Ethylene Terephthalate) (1) (HFIP-806M)
    • Poly(Ethylene Terephthalate) (2) (HFIP-606M)
    • Poly(Butylene Terephthalate) (1) (Eluent: HFIP)
    • Polyacetal (1) (HFIP-806M)
    • Polyacetal (2) (Delrin)
    • Poly(Methyl Methacrylate) Standards (3) (HFIP-606M)
    • Polyacetal (3) (HFIP-606M)
    • Poly(Vinyl Alcohol) (1) (HFIP Eluent)
    • Ethylene Vinyl Acetate Copolymer (2) (Eluent: HFIP)
    • Comparison of Analysis of PMMA with Down-sized GPC Columns
    • Polyacetal (4) (LF-404)
    • Poly(Ethylene Terephthalate) (3) (LF-404)
  • Hydrophobic Polymers: Other Eluents
    • Polystyrene Standards (10) (Toluene Eluent)
    • Silicone Oil (Toluene Eluent)
    • Dimethyl Silicone Oil (Toluene Eluent)
    • Rubber (Toluene Eluent)
    • Polystyrene Standards (19) (DMAc Eluent)
    • Polystyrene Standards (20) (NMP Eluent)
    • Poly(-N,N-Dimethyl Aminomethyl Styrene) (2) (NMP Eluent)
    • N-Methoxymethyl Nylon (Methanol Eluent)
    • Melamine Formaldehyde Resin (2) (Mixed Solvent Eluent)
    • Epoxy Resin (3): Mixed Solvent Eluent
    • p-Cresol Formaldehyde Resol Resin (Mixed Solvent Eluent)
    • Phenol Formaldehyde Novolac Resin (Mixed Solvent Eluent)
    • Polyamic Acid (LF-804)
    • Poly(Ethylene Oxid) and Poly(Ethylene Glycol) standards (2) (LF-804)
  • Hydrophobic Polymers: High Temperature GPC
    • Calibration Curves for UT-800 Series
    • Calibration Curves for HT-800 Series
    • Calibration Curves for HT-806M and AT-806M
    • Features of UT-800 Series
    • HDPE Sample-A (1)
    • HDPE Sample-A (2)
    • HDPE Sample-B (1)
    • HDPE Sample-B (2)
    • LDPE Sample-C
  • Additives in Hydrophobic Polymers
    • Phthalates and Alkylbenzenes (1) (KF-801)
    • Phthalates and Alkylbenzenes (2) (KF-401HQ)
    • Phthalates
    • Alkylbenzenes (6) (RP18-613)
    • Effect of Flow Rate on Plate Number
    • Recycle Analysis of Impurity in Resin
    • Analysis of Antioxidant in Polystyrene (GPC/MS)
    • Analysis of Irganox 1010 in Cup of Instant Noodles (Styrene Foam)
    • Simultaneous Analysis of Various Additives of Polymer in Polystyrene (GPC/MS)
  • Hydrophobic Oligomers
    • PEEK Oligomer
    • PET Oligomer
    • Epoxy Resin Oligomer
    • Effect of Flow Rate on Elution Pattern
    • Effect of Column Length on Elution Pattern
  • Hydrophilic Polymers
    • Pullulan Standards (1) (SB-804 HQ)
    • Pullulan Standards (2) (GF-310 HQ)
    • Pullulan Standards (3) (GF-710 HQ)
    • Pullulan Standards (4) (Effect of Flow Rate)
    • Pullulan Standards (5) (Effect of Temperature)
    • Pullulan Standards (7) (GS-220 HQ)
    • Poly(Ethylene Glycol) Standards (6) (DE-613)
    • Poly(Ethylene Glycol) Standards (7) (SB-2002.5)
    • Poly(Ethylene Glycol) Standards (8) (SB-802.5 HQ)
    • Poly(Ethylene Glycol) Standards (9) (GS-220 HQ)
    • Poly(Ethylene Oxide) and Poly(Ethylene Glycol) Standards
    • Selection of Column (1) (Sodium Poly(Styrene Sulfonate))
    • Selection of Column (2) (Sodium Polyacrylate)
    • Selection of Column (3) (Poly(Sodium Methacrylate))
    • Hydroxyethyl Cellulose (2) (SB-805 HQ)
    • Dextran (1) (SB-805 HQ)
    • Dextran (2) (DMSO Eluent)
    • Dextran (3) (SB-806M HQ)
    • Dextran Sulfate
    • Poly(Ethyleneimine)
    • Polyacrylamide (1) (SB-805 HQ)
    • Poly(Vinyl Alcohol) (2) (Aqueous Eluent)
    • Poly(Vinylpyrrolidone) (3) (NaNO 3 /CH 3 CN Eluent)
    • Sodium Polyacrylate (1) (SB-803 HQ + SB-802 HQ)
    • Sodium Polyacrylate (2) (GSM-700 7E)
    • Poly(Sodium Methacrylate)
    • Sodium Poly(Styrene Sulfonate) (1) (GF-510 HQ)
    • Sodium Poly(Styrene Sulfonate) (2) (GF-7M HQ)
    • IInfluence of Retention Time of PEG by Column Temperature
    • Influence of Salt Concentration on Retention Time of PEG with GF-310 HQ Column
    • Comparison of Chromatogram of PEG Mixtures
    • Influence of Analysis of PEG Mixtures with GF-310 HQ by Composition of Eluent
    • Influence of Analysis of PEG Mixtures with GF-320 HQ by Composition of Eluent
    • Polyacrylamide (2) (KS-807, SB-806 HQ)
    • Polyacrylamide (3) (Comparison with SB-807 HQ and SB-806 HQ)
    • Effect of Sample Load (Pullulan)
  • Cationic Polymers
    • Measurement of Molecular Weight Distribution of Poly(Allylamine)Hydrochloride
    • Measurement of Mmolecular Weight Distribution of Poly(Ethylene Imine)
    • Measurement of Molecular Weight Distribution of Poly(Diallyl Dimethyl Ammonium Chloride)
  • Recycle Analysis of Polymers
    • Recycle Analysis of Lauryl Stearyl Thiodipropionate
    Anions and Cations
    Column Switching
    Other Applications
    NP & RP (Normal & Reversed Phase)
    SEC (Size Exclusion Chromatography)
    Saccharides & Organic Acids Analysis
    IEX (Ion Exchange Chromatography)
    IC (Ion Chromatography)
    HIC (Hydrophobic Interaction Chromatography)
    Affinity Chromatography
    Chiral Separation
    LC/MS & Rapid Analyses of Medicines
    Exclusive Use
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    Column Switching
    GPC Clean-up
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    Pullulan Standards (5) (Effect of Temperature)

    Pullulan standards were analyzed using OHpak SB-2002.5 (a column for GFC separation).



    Sample : Pullulan
    Column       : Shodex OHpak SB-2002.5 (20.0mmID*300mm)
    Eluent       : 0.02% NaNO3 aq.
    Flow rate    : 3.0mL/min
    Detector     : Shodex RI
    Column temp. : (A); Room temp., (B); 50°C, (C); 70°C, (D); 80°C
    

     
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