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Asahipak ODP-50 columns with C18 groups: a polymer-based RP column for many applications. Silanol-free and stable until pH 13. Usable in 100% water and organic solvent. Asahipak ODP-50 columns with C18 groups The robust polymer-based RP column for many general applications Product information Asahipak ODP-50 The Shodex Asahipak ODP-50 series are columns for reversed phase chromatography. These columns are packed with spherical porous polymer having octadecyl (C18) groups, and perform similar to general silica-based C18 ODS columns. These columns are compatible with not only water or polar organic solvents, but also the wide pH range between 2 to 13. There are ideal for LC-MS/MS analysis. Relatively large pore size of 250 A is suitable for the analysis of amino acids, peptides, and proteins Usable in a wide pH range from pH 2 to 13 Usable in 100 % water and buffer solution Best used for the analysis of basic substances Fulfills USP L67 requirements Also available as Asahipak C4P-50 with C4 groups Also suitable for evaporative light scattering detector (ELSD), corona charged aerosol detector (CAD), and mass spectrometry (LC-MS/MS) Over 60% of all HPLC applications are Reversed Phase (RP) separations with C18 functional groups. Spherical silica particles proved their use as base material in HPLC columns for many years. To minimize the negative effects of residual silanol groups on the surface, researchers tried end-capping or polymeric coating of the particle and developed hybrid materials. Here we introduce an alternative for these ODS (octadecyl-bonded silica) RP columns: A polymer particle (for example polyvinyl alcohol) with 5 μm particle size is modified with C18 alkyl chains as functional groups and used as stationary phase. This HPLC column has greater long-term pH stability from 2 to 13. It contains no silanol groups and provides higher resolution for basic substances, for example drugs with tertiary amines (no peak tailing due to unwanted interactions). Also the polarity of the polymer gel is slightly more hydrophilic which gives more retention of polar compounds at the beginning of the chromatogram. The polymer-based RP material can be used in 100% water or buffer and a variety of organic solvents (isocratic and gradient separation). It has a pore size of 250 Å which makes it suitable for the analysis of proteins and peptides. The very low bleeding is advantageous for the hyphenation to mass spectrometry (MS) or particle-sensitive light scattering detectors. A C18 column with a stationary phase made of polymer offers more than double the life time compared to silica, because it is chemically more resistant. Shodex is specialized in polymer-based packing material for Reversed Phase chromatography. The modern column Asahipak ODP-50 (octadecyl-bonded polymer) combines all the advantages of a polymeric RP-C18 column. It is used for small organic molecules, pharmaceuticals, pesticides, hormones, proteins, vitamins and basic drugs. Product overview Product code Product name Separation Functional group Gel material Plates per column Particle size Pore size Size (ID x length) Housing material F7620009 Asahipak ODP-50 2D RP C18 Polyvinyl alcohol ≥ 5,000 5 µm 250 Å 2.0 x 150 mm steel F6713001 Asahipak ODP-50G 2A RP C18 Polyvinyl alcohol (guard) 5 µm - 2.0 x 10 mm steel F6710023 Asahipak ODP-50 4B RP C18 Polyvinyl alcohol ≥ 2,500 5 µm 250 Å 4.6 x 50 mm steel F7620004 Asahipak ODP-50 4D RP C18 Polyvinyl alcohol ≥ 9,000 5 µm 250 Å 4.6 x 150 mm steel F7620003 Asahipak ODP-50 4E RP C18 Polyvinyl alcohol ≥ 14,000 5 µm 250 Å 4.6 x 250 mm steel F6710022 Asahipak ODP-50G 4A RP C18 Polyvinyl alcohol (guard) 5 µm - 4.6 x 10 mm steel F7620002 Asahipak ODP-50 6D RP C18 Polyvinyl alcohol ≥ 9,000 5 µm 250 Å 6.0 x 150 mm steel F7620001 Asahipak ODP-50 6E RP C18 Polyvinyl alcohol ≥ 14,000 5 µm 250 Å 6.0 x 250 mm steel F6710001 Asahipak ODP-50G 6A RP C18 Polyvinyl alcohol (guard) 5 µm - 6.0 x 10 mm steel F6820001 Asahipak ODP-50 10E RP C18 Polyvinyl alcohol ≥ 10,000 5 µm 250 Å 10.0 x 250 mm steel F7620008 Asahipak C4P-50 4D RP C4 Polyvinyl alcohol ≥ 6,000 5 µm 250 Å 4.6 x 150 mm steel F6710003 Asahipak C4P-50G 4A RP C4 Polyvinyl alcohol (guard) 5 µm - 4.6 x 10 mm steel Product details Product name Maximum pressure Usual flow rate Maximum flow rate Temperature range pH range Salt concentration Shipping solvent USP Info Asahipak ODP-50 2D 12 MPa (120 bar) 0.2 mL/min 0.4 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50G 2A - - - 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50 4B 5.0 MPa (50 bar) 0.4 to 0.6 mL/min 1.5 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50 4D 15 MPa (150 bar) 0.5 to 0.8 mL/min 1.5 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50 4E 15 MPa (150 bar) 0.5 to 0.8 mL/min 1.5 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50G 4A - - - 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50 6D 15 MPa (150 bar) 0.5 to 1.0 mL/min 2.5 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50 6E 15 MPa (150 bar) 0.5 to 1.0 mL/min 2.5 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50G 6A - - - 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak ODP-50 10E 15 MPa (150 bar) 1.5 to 2.0 mL/min 3.0 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 L67 Carbon load 17% Asahipak C4P-50 4D 15 MPa (150 bar) 0.5 to 0.8 mL/min 1.0 mL/min 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 - Carbon load 6% Asahipak C4P-50G 4A - - - 4 to 60°C 2 to 13 ≤ 0.1 M H2O/ACN = 35/65 - Carbon load 6% Product info Asahipak ODP-50 2D | 2.0 x 150 mm Out of stock Asahipak ODP-50G 2A | 2.0 x 10 mm guard Out of stock Asahipak ODP-50 4B | 4.6 x 50 mm Out of stock Bestseller Asahipak ODP-50 4D | 4.6 x 150 mm Price €975.00 Excluding VAT Bestseller Asahipak ODP-50 4E | 4.6 x 250 mm Price €1,030.00 Excluding VAT Asahipak ODP-50G 4A | 4.6 x 10 mm guard Price €542.00 Excluding VAT Asahipak ODP-50 6D | 6.0 x 150 mm Out of stock Asahipak ODP-50 6E | 6.0 x 250 mm Price €1,056.00 Excluding VAT Asahipak ODP-50G 6A | 6.0 x 10 mm guard Out of stock preparative Asahipak ODP-50 10E | 10.0 x 250 mm Out of stock Asahipak C4P-50 4D | 4.6 x 150 mm Price €975.00 Excluding VAT Asahipak C4P-50G 4A | 4.6 x 10 mm guard Out of stock Download documents Shodex Technical Article 14_Analysis of Functional Ingredients in Dietary Supplement (pdf) Shodex Application Note Polymer RP-C18 Asahipak ODP-50 (pdf) Operation Manuals: Operation manuals for each column series can be found on our Japanese website. Please click the following link to be directed to the Operation Manual Database. https://www.shodex.com/en/download Product instruction Eluents: It is possible to use pure water, methanol and acetonitrile 100 % each, and any proportion of these solvents. Acetonitrile is recommended in case of low resolution of aromatic substances with methanol containing eluent. It is possible to use buffer agents like phosphoric salt, acetate salt and Tris-HCI. And neutral salts such as NaCl, KCI, Na2SO4 and acids such as trifluoroacetic acid are also usable. Cleaning: Elution characteristics of a column may change considerably after long, repeated usage, due to the accumulation of sample microadsorbents on the packing material surface. In such cases, remove guard column and set analysis column with reverse direction with its flow direction, cleaning the column with 50 mL or more of acetonitrile or methanol may be effective for recovery of column efficiency. Guard column should be cleaned alone with the same way. The flow rate should be applied lower than 0.5 mL/min. Applications Please take a look at the following list of example application data and chromatograms obtained with our columns. These links will redirect you to our Japanese website. General Comparison of Silica-based Column with Polymer-based Column Durability of ODP-50 Column with Alkaline Eluent Effects of Eluent pH (ODP-50) Effects of pH of Eluent on Elution Volume and Plate Number (ODP-50) Effects of pH of Eluent on Sensitivity of Barbital (ODP-50 4D) Preservative 2-Phenoxyethanol (ODP-50 4D) Preservatives (ODP-50 4D) Other Anilines (ODP-50 6D) Aliphatic Alcohols (3) (ODP-50 6D, C4P-50 6D) Berberine in Japanese Goldthread (ODP-50 4D) Analysis of Chelating Agents (ODP-50 4D) DNPH Derivative Aldehydes (ODP-50 4D) Short-Chain Amylose (ODP-50 6D) Analysis of Residual ε-Caprolactam in Nylon 6 (ODP-50 4D) Bioanalysis Hydroxyeicosatetraenoic Acid Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D) Hydroxyeicosatetraenoic Acids (ODP-50 6D) Leukotrienes (ODP-50 6D) Leukotrienes Produced by Polymorphonuclear Leukocyte of Rat (ODP-50 6D) Antibiotics Analysis of Azithromycin According to JP Method (ODP-50 4E) Analysis of Azithromycin According to USP-NF Method (ODP-50 4E) Analysis of Azithromycin Injection According to USP-NF Method (ODP-50 4D) Antibiotics (1) (ODP-50 4D) Analysis of Macrolide Antibiotics and Derivatives (ODP-50 4D) Tetracycline Derivative Antibiotics (ODP-40 4D) Drugs Analysis of Formoterol Fumarate Dihydrate According to Pharmacopeia Method (ODP-50 4D) Anti Cancer Drugs (ODP-50 4D) Anti Inflammatory Drugs (1) (ODP-50 4E) Anti Inflammatory Drugs (2) (ODP-50 4E) Antipyretic Analgesics (ODP-50 4D) Barbiturates (ODP-50 4D) Basic Drugs : Effects of Eluent pH Basic Drugs : Effects of Ion Concentration Basic Drugs : Under Neutral Condition Cinchona Alkaloids (ODP-50 6D) Diuretics (1) (ODP-50 4D) Drugs (ODP-50 4D) Indomethacin in Serum (ODP-50 6E) Local Anesthetics (1) (ODP-50 4D) Procainamide in Serum (ODP-50 4D) Procaine and Procaineamide (ODP-50 4D) Saikosaponins (ODP-50 6D) Tetracaine (ODP-50 4D) Peptide Analysis of Glutathiones (By Post-column Reaction Method) (ODP-50 6E) Analysis of Oligopeptides with Acidic and Alkaline Eluent ( ODP-50 6D) Angiotensins (ODP-50 6D) Basic Peptides (ODP-50 6D) Oligopeptides (1) (ODP-50 6D) Oligopeptides (2) (ODP-50 6D) Opioid Peptides (1) (By Post-column Reaction Method) (ODP-50 6D) Opioid Peptides (2) (By Pre-column Reaction Method) (ODP-50 6D) Proteins and Peptides (ODP-50 6D) Recovery of Peptides (ODP-50 6D) Tryptic Digest of Myoglobin (2) (ODP-50) Amin Analysis of Histamine by On-Column Derivatization (ODP-50 4E) Analysis of Polyamines by On-Column Derivatization (ODP-50 4D) Catecholamines and Metabolites (1) (ODP-50 6D) Polyamine and Acetylated Derivatives (ODP-50 4E) Scopolamine and Atropine (ODP-50 4D) Food Analysis of Unsaturated Fatty Acids in Supplements (ODP-50 4D) Analysis of α-Linolenic Acid in Edible Blended Oil (ODP-50 4D) 5-(Hydroxymethyl) Furfural (5-HMF) in Fruit Juice (ODP-50 6D) Ethoxyquin (ODP-50 4E) Fat-Soluble Vitamins (1) (ODP-50 4E) Food Yellow No.4 (Tartrazine) (ODP-50 4D) Glycyrrhizin in Licorice (ODP-50 6D) Histidine and 1-Methylhistidine (ODP-50 6D) Unsaturated Fatty Acids (ODP-50 4D) Vitamin B6 (1) (ODP-50 6D) Vitamin D (ODP-50 6E)

Polymer-based HILIC columns: Asahipak NH2P-50 with amino groups and the HILICpak series with amino, quaternary ammonium, carboxyl and diol groups. For the analyis of polar compounds like sugars, sweeteners, water-soluble vitamins, pesticides (glyphosate), drugs, peptides. HILIC (hydrophilic interaction liquid chromatography) Polymer-based columns for the analysis of polar substances in drugs, food or biological samples Product overview Asahipak NH2P-50 series Polymer-based HILIC column with amino groups (-NH2) For polar substances Suitable for saccharides analysis using HILIC mode Polymer-based packing material provides excellent chemical stability and minimum deterioration over extended time period Easily regenerated by washing in an alkaline solution Appropriate for ELSD, CAD, LC-MS/MS Fulfills USP L82 requirement Asahipak NH2P-50 series HILICpak VG-50 / HILICpak VG-25 Polymer-based HILIC columns with tertiary amino (-NR2) groups Suitable for saccharide analysis using HILIC mode Recovers reducing saccharides with high ratio (improved results for galactose, mannose, arabinose and xylose in comparison to Asahipak NH2P-50 series) Best for melamine analysis Appropriate for ELSD, CAD, LC-MS/MS Steel and PEEK housings to avoid interaction Particle size 5 µm or 2.5 µm HILICpak VG-50/VG-25 (amino) HILICpak VT-50 Polymer-based HILIC columns with quaternary ammonium (-NR3+) groups Best for glyphosate analysis and phosphorylated sugars Suitable for anionic substances (especially phosphate compounds) analysis using HILIC mode Use of some eluents add ion exchange mode Polymer-based packing material provides excellent chemical stability and minimum deterioration over an extended time period Suitable for LC-MS/MS analysis HILICpak VT-50 (quat. ammonium) HILICpak VC-50 Polymer-based HILIC columns with carboxyl (-COO-) groups Modified carboxyl group is suitable for cationic substance analysis including amines The dominant separation mode is RP or IEX rather than HILIC mode Best for aminoglycoside antibiotics, histamine/histidine, monoamine neurotransmitters and more Suitable for LC-MS/MS analysis HILICpak VC-50 (carboxyl) HILICpak VN-50 Polymer-based HILIC columns with diol (-OH) groups Best for oligosaccharides and oligonucleotides Suitable for oligomer separation which is not possible by SEC column or conventional HILIC columns The modified diol groups on the packing material create the HILIC mode HILICpak VN-50 (diol)

The ODP2 HP columns are special polymer-based reversed phase columns for the analysis of small molecules in the presence of a protein matrix. They offer enhanced retention of polar substances and are ideal for LC-MS/MS. ODP2 columns with small pores The robust polymer-based RP column for small molecules in protein matrix Catalog Product Overview (pdf) Product information ODP2 HP columns The Shodex ODP2 HP series offers polymer–based columns for reversed phase chromatography. The efficiency of ODP2 HP columns is improved over most resin-based columns and is comparable with that of silica-based ODS columns. They don't contain C18 functional groups, the separation comes from the particle itself. ODP2 HP has a better retention of highly polar substances compared to most general purpose silica-based C18 columns. Silica-based C18 ODS columns are susceptible to protein adsorption, resulting in degradation of the column. ODP2 HP is designed to exclude protein and thus, ODP2 HP can be used for analysis of drugs in biological samples containing protein without rapid column deterioration. As ODP2 HP can be used with low salt concentration without loss of peak shapes, it is excellent for LC/MS analysis. Provides a large theoretical plate number nearly twice as much as generally available polymer-based reversed phase columns do Offers enhanced retention of high polar substances compared to ODS columns Suitable for the analysis of small molecules such as pharmaceuticals in the presence of protein matrix Ideal for LC/MS analysis of high polar compounds Fulfills USP L39 requirements The material is completely silanol-free and therefore proteins are not adsorbed in the column. Product overview Product code Product name Separation Functional group Gel material Plates per column Particle size Pore size Size (ID x length) Housing material F7622004 ODP2 HP-2B RP - Polyhydroxy-methacrylate ≥ 3,000 5 µm 40 Å 2.0 x 50 mm steel F7622005 ODP2 HP-2D RP - Polyhydroxy-methacrylate ≥ 7,000 5 µm 40 Å 2.0 x 150 mm steel F6714011 ODP2 HPG-2A RP - Polyhydroxy-methacrylate (guard) 5 µm - 2.0 x 50 mm steel F7622001 ODP2 HP-4B RP - Polyhydroxy-methacrylate ≥ 3,500 5 µm 40 Å 4.6 x 50 mm steel F7622002 ODP2 HP-4D RP - Polyhydroxy-methacrylate ≥ 10,000 5 µm 40 Å 4.6 x 150 mm steel F7622003 ODP2 HP-4E RP - Polyhydroxy-methacrylate ≥ 17,000 5 µm 40 Å 4.6 x 250 mm steel F6714010 ODP2 HPG-4A RP - Polyhydroxy-methacrylate (guard) 5 µm - 2.0 x 50 mm steel Product details Product name Maximum pressure Maximum flow rate Temperature range pH range Salt concentration Shipping solvent USP ODP2 HP-2B 12 MPa (120 bar) 0.3 mL/min 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 ODP2 HP-2D 15 MPa (150 bar) 0.3 mL/min 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 ODP2 HPG-2A - - 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 ODP2 HP-4B 15 MPa (150 bar) 1.5 mL/min 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 ODP2 HP-4D 15 MPa (150 bar) 1.5 mL/min 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 ODP2 HP-4E 15 MPa (150 bar) 1.0 mL/min 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 ODP2 HPG-4A - - 20 to 60°C 2 to 12 ≤ 0.1 M H2O/ACN=55/45 L39 Product info ODP2 HP-2B | 2.0 x 50 mm ODP2 HP-2D | 2.0 x 150 mm ODP2 HPG-2A | 2.0 x 10 mm guard ODP2 HP-4B | 4.6 x 50 mm ODP2 HP-4D | 4.6 x 150 mm ODP2 HP-4E | 4.6 x 250 mm ODP2 HPG-4A | 4.6 x 10 mm guard Product instruction Eluents: ODP2 HP may be used with water, acid, base and aqueous salt solutions including most popular buffers, acetonitrile, methanol and mixtures of these components. Phosphoric Acid, Formic Acid, Acetic Acid, and Trifluoroacetic Acid Ammonia Phosphate Buffer, Formate Buffer, Acetate Buffer, and Carbonate Buffer Methanol, Acetonitrile Precautions 1) Eluent should be in the pH range of 3~12. 2) The total concentration of acid, base, and salt should be 100 mM or less. Generally, a range of 1~50 mM is recommended. 3) When adding acetonitrile or methanol to the aqueous salt solution, confirm there is no salt precipitation before use. 4) Nonpolar organic substances such as hexane or toluene cannot be used. Download documents Shodex Technical Notebook No.6 (ODP2, high performance RP for LC-MS) (pdf) Operation Manuals: Operation manuals for each column series can be found on our Japanese website. Please click the following link to be directed to the Operation Manual Database. https://www.shodex.com/en/download Applications Please take a look at the following list of example application data and chromatograms obtained with our columns, These links will redirect you to our Japanese website. General Advantage of ODP2 HP (2) High Retention of High Polarity Substances Advantage of ODP2 HP (3) High Removal Ability of Protein Advantage of ODP2 HP (4) Ability for Use with Low Salt Concentrations Advantage of ODP2 HP (5) Alkali Durability Analysis of Short Amines without Using Ion Pair Reagent Effects of Column Temperature (ODP2 HP) Effects of Flow Rate (ODP2 HP) Effects of Organic Solvent in Eluent (ODP2 HP) Effects of Sample Injection Volume (ODP2 HP) Effects of Sample Loads (ODP2 HP) Small molecules in biological matrix Analysis of Anticonvulsant in Serum (ODP2 HP) Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-4D) Analysis of Polysorbate 80 in Antibody Drugs (ODP2 HP-4D) Direct Analysis of Acetaminophen in Serum (ODP2 HP) Direct Analysis of Risperidone in Urine (ODP2 HP) Drug Antibody Ratio Analysis of Antibody-Drug Conjugate (ODP2 HP-4B) Imidazoles (ODP2 HP-4E) Short Amines (ODP2 HP) Simultaneous Analysis of Multi Symptom Cold Formula (ODP2 HP) Simultaneous Analysis of Multi Symptom Cold Formula in Serum (ODP2 HP) LC-MS/MS Influence of Proteins in LC/MS Analysis (ODP2 HP-2B) LC/MS Analysis of Barbital in BSA (ODP2 HP-2B) LC/MS Analysis of Drugs in BSA (1) (ODP2 HP) LC/MS Analysis of Drugs in BSA (2) (ODP2 HP) LC/MS Analysis of Drugs in Control Serum (ODP2 HP) LC/MS Analysis of Poloxamer 188 in the Presence of IgG (ODP2 HP-2B) LC/MS analysis of Polysorbate 20 in Antibody Drug Complex Preparation (ODP2 HP-2B） LC/MS Analysis of Statins (ODP2 HP-2B) LC/MS Analysis of Tween Type Surfactants in IgG (ODP2 HP-2D) LC/MS/MS Analysis of Sulfamic Acid According to Japanese Testing Methods for Fertilizers (ODP2 HP-2D) LC/MS/MS Analysis of Tricyclic Antidepressants in BSA (ODP2 HP) Reproducibility in LC/MS Analysis (ODP2 HP)