, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Opioid Peptides (2) (By Pre-column Reaction Method)

Opioid peptides were analyzed using Asahipak ODP-50 6D by pre-column reaction method.

Sample :
1. Tyr-Gly-Gly-Phe
2. ME, Met-Enkephalin
3. LE, Leu-Enkephalin
4. LE-Arg
5. α-EDP, α-Endorphin
6. LE-NH2
7. DNP 1-7
8. [D-Ala2] LE-NH2
9. ME-Arg-Gly-Leu
10. γ-EDP
11. ME-Arg-Phe
12. DNP 1-8
13. β-NED, β-Neoendorphin
14. α-NED, β-NED-Lys
15. DNP 1-9
16. DNP 1-10
17. DNP-B



Column : Shodex Asahipak ODP-50 6D (6.0mmI.D. x 150mm)
Eluent : (A); 0.1M Borate buffer(pH9.5)/H2O/CH3CN=10/39/1 (B); 0.1M Borate buffer(pH9.5)/H2O/CH3CN=2/3/5 Gradient:(A) to (B)
Flow rate : (0 to 25min); 0.8mL/min, (25 to 55min); 1.0mL/min, (after 55min); 0.8mL/min
Detector : Fluorescence(Ex. 330nm, Em. 430nm)
Column temp. : Room temp.

Guo-Qing Zhang et al., Chem. Pharm. Bull., 39 (1991) 126.