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Whey Proteins

Whey proteins were analyzed using RSpak RP18-415 (a column for reversed phase chromatography).

Sample: Whey protein
1. γ-Lactoalbumin
2. β-Lactoglobulin A
3. β-Lactoglobulin B



Column : Shodex RSpak RP18-415 (4.6mmI.D. x 150mm)
Eluent : (A); 0.1% TFA aq. (B); 0.1% TFA in CH3CN Linear gradient: 0 to 30min, 30% (B) to 50% (B)
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : Room temp.