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Analysis of Glutathiones (By Post-column Reaction Method)

Reduced glutathione (GSH) and oxidized glutathione (GSSG) are separated with using polymer based reversed phase column, Asahipak ODP-50 6E .

Sample : 5ppm each,
1. Glutathione , GSH (MW: 307.33)
2. Glutathione disulfide, GSSG (MW: 612.63)



Column : Shodex Asahipak ODP-50 6E (6.0mmI.D. x 250mm)
Eluent : 50mM Sodium phosphate buffer(pH2.5) Reagent : 50mM o-Phthalaldehyde in CH3OH/200mM Sodium phoshate buffer(pH12.0)/ 2-Mercaptoethanol=50/450/1
Flow rate : (
Eluent); 0.5mL/min, (reagent); 0.3mL/min
Detector : Fluorescnce(Ex. 340nm, Em. 420nm)
Column temp. : 40°C