1. 5mM tartaric acid + 1mM dipicolinic acid Eluent + 1.5g/L boric acid The eluent of 5mM tartaric acid + 1mM dipicolinic acid + 1.5g/L boric acid is the one generally used for the analysis of cations such as Na+, NH4+, K+, Ca2+ and Mg2+ which are most commonly contained in actual samples. Although Mn2+ elute just after K+, all the other transition metal ions elute at Vo. Separation can be adjusted by changing the eluent concentration as follows : * Tartaric acid------5mM is used most commonly. The use of higher concentrations such as 6mM to 7mM, causes cations to elute faster. * Dipicolinic acid---1mM is used most commonly. It is added to make divalent cations elute faster. At a higher temperature such as 50 °C, Sr2+, Ba2+ elute faster.
(Preparation of eluent) 1) Put 0.750g of reagent grade L(+)tartaric acid, 0.167g of reagent grade dipicolinic acid and 1.5g of reagent grade boric acid into a volumetric flask. 2) Dilute with purified water to make up a 1 liter solution. 3) Dissolve applying ultrasonic vibration. 4) Pass through a 0.2μm membrane filter before use. (Remark) Since tartaric acid deteriorates so fast, this eluent should be prepared just before the use.
2. Addition of crown ether The separation between Na+ and NH4+ can be largely improved by adding crown ether in the eluent of 5mM tartaric acid + 1mM dipicolinic acid + 1.5g/L boric acid. For example, Na+ and NH4+ in serum of which concentration ratio is 1 : 300 can be separated well.
3. Addition of acetoritorile Acetonitorile can be added up to 70% in nitric acid aqueous solution. Therefore, in adition to organic cations, ethanolamine and short chain alkylamines which could be analysed using the old version column(YF-421), long chain alkylamines can also be analysed using IC YK-421.