, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Comparison with ODS Column (1)

The comparison of Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column) and an ODS column when they were used for the measurement of POE distribution of octylphenol ethoxylate is shown here. When GF-310 HQ was used, good chromatograms were obtained using 35 and 40% acetonitrile concentration. On the other hand, when an ODS column was used, POE distribution can be measured using 55% acetonitrile concentration but the separation is not sufficient.

Sample :
Octylphenol ethoxylate



Column : Shodex Asahipak GF-310 HQ (7.5mmI.D. x 300mm)
Eluent : CH3CN/H2O
Flow rate : 0.6mL/min
Detector : UV(280nm)
Column temp. : 30°C

Column : ODS
Column from the other manufacturer
Eluent : CH3CN/H2O
Flow rate : 0.8mL/min
Detector : UV(280nm)
Column temp. : 30°C