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Benzalkonium Chloride (2) (GF-310 HQ)

Asahipak GF-310 HQ (an Aqueous/Organic (multi-solvent use) SEC column) was used to analyze a cationic surfactant, benzalkonium chloride. Two mobile phase salts, 50 mM sodium chloride and 50 mM sodium nitrate, mixed with ACN were tested. Both solvents provided good analytical performances by suppressing ionic interactions. It should be noted that the sodium nitrate has UV absorbance, thus if UV detector was used, the background level will become high. This may result in lower sensitivity compared to using sodium chloride as a salt.

Sample: Benzalkonium chloride 0.2%, 20μL



Column : Shodex Asahipak GF-310 HQ (7.5mmI.D. x 300mm)
Eluent : (A); 50mM NaCl aq./CH3CN=50/50 (B); 50mM NaNO3 aq./CH3CN=50/50
Flow rate : 0.6mL/min
Detector : UV(254nm)
Column temp. : 40°C