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Cyanate and Carbamyl Phosphate

Cyanate and carbamyl phosphate were analyzed using Asahipak ES-502N 7C (a column for weak cation exchange chromatography).

Sample : 20μM each, 50μL
1. Carbamyl phosphate
2. Cyanate




Column : Shodex Asahipak ES-502N 7C (7.5mmI.D. x 100mm)
Eluent : 0.15M NaCl aq. Reagent : (I); 0.1M acetate buffer(pH3.0) containintg 2%(w/v) Phenol and 10mg/dL Sodium nitropreusside (II); 1.0%(w/v) Sodium hydroxide containing 0.1%(w/v) Sodium hypochlorite (for hydrolysis under acidic condition/indophenol reaction)
Flow rate : 1.0mL/min
Detector : VIS(635nm)
Column temp. : 20°C

I.Koshiishi et al., Anal.Sci., 2 (1986) 81.