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Amides (2) (Comparison of Reversed Phase Columns)

Three reversed phase columns RSpak DM-614, DE-613 and DS-613 were used for the analysis of alkylamides. For this sample, DM-614 is not suited because of too high polarity of its packing material, and, DS-613 is also not suited because of too long elution time. DE-613 is best suited for this sample.

Sample :
1. Acetamide
2. Propionamide
3. Butylamide
4. Valeramide



Columns : (A); Shodex RSpak DM-614, (B); DE-613, (C); DS-613 (6.0mmI.D. x 150mm each)
Eluent : H2O/CH3OH=70/30
Flow rate : 1.0mL/min
Detector : UV(210nm)
Column temp. : 35°C