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Trypsin and α-Chymotrypsin

The pIs of trypsin and α-chymotrypsin are approximately 8.1-8.6 and 10.1-10.6 respectively. These basic proteins can be separated well with the IEC CM-825 (a column for weak cation exchange chromatography).

Sample : 30μL
1. Trypsin 0.5%
2. α-Chymotrypsin 0.25%


Column : Shodex IEC CM-825 (8.0mmI.D. x 75mm)
Eluent : (A); 20mM Sodium phosphate buffer(pH7.0) (B); (A) + 0.5M NaCl Linear gradient: 0min to 60min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)
Column temp. : Room temp.