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High Sensitive Analysis of Metal Bonded Amino Acids

High sensitive analysis of metal bonded amino acids was performed using GS320A-M8E (0.8mm I.D. x 250mm) and ICP-MS. GS320A-M8E is a μcolumn of Asahipak GS-320 HQ . Using the micro column, It is possible to reduce the injection volume and eluent to 1/200 and 1/100 compared with the case where a conventional column is used, respectively.

Sample :
MeSeCys (Methylselenocysteine)
SeMet (Selenomethionine)



            (Left)                                         (Right) 
LC        : capillary HPLC                                 normal HPLC
          (Tubing: 0.03mmI.D. fused-silica capillary tube) (Tubing: 0.25mmI.D. PEEK tube)

Columns : Shodex GS320A-M8E Shodex Asahipak GS-320 HQ (0.8mmI.D. x 250mm) (7.5mmI.D. x 300mm)
Eluent : 50mM Ammonium acetate(pH6.5) 50mM Ammonium acetate(pH6.5)
Flow rate : 5.5μL/min 500μL/min
Detector : ICP-MS Se at m/z=82 ICP-MS Se at m/z=82

Courtesy of Dr. Yasumitsu Ogra, Graduate School of Pharmaceutical Sciences, Chiba University