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LC/MS Analysis of Citrulline (NH2P-40 2D)

Citrulline, an α-amino acid, was analyzed by Asahipak NH2P-40 2D a polymer-based amino column under HILIC mode. Hydrophilic compounds are retained at a high concentration of acetonitrile under HILIC mode conditions. This is advantageous for high sensitivity analysis by LC/MS.

Sample : 5μL
L-Citrulline 20ng/mL (in H2O/CH3CN=25/75)



Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)
Eluent : 20mM HCOONH4 aq./CH3CN=25/75
Flow rate : 0.2mL/min
Detector : ESI-MS/MS MRM
Column temp. : 30°C