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Nucleobases (1) (GS-320 HQ)

Nucleobases were analyzed using Asahipak GS-320 HQ (a multimode column).

Sample : 50μg/mL each, 20μL
1. Cytosine
2. Adenine
3. Guanine
4. Uracil
5. Hypoxanthine
6. Thymine



Column : Shodex Asahipak GS-320 HQ (7.5mmI.D. x 300mm)
Eluent : 50mM NaH2PO4 aq./50mM H3PO4 aq.=500/380
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 30°C