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Guanine and Tryptophan (DM-614, NN-814 and DE-613)

Guanine and tryptophan were analyzed using three
Columns for reversed phase chromtography, RSpak DM-614, NN-814 and DE-613.

Sample : 1. Guanine, 2. Tryptophan

Columns : (left); Shodex RSpak DM-G 4A (4.6mmI.D. x 10mm) + DM-614 (6.0mmI.D. x 150mm) (Center); Shodex RSpak NN-G (6.0mmI.D. x 50mm) + NN-814 (8.0mmI.D. x 250mm) (Right); Shodex RSpak DE-G 4A (4.6mmI.D. x 10mm) + DE-613 (6.0mmI.D. x 150mm)

Eluent : (Left); 0.3M K2HPO4(pH8.6) (Center); 0.1M NaH2PO4(pH3.0 adjusted by H3PO4) (Right); 0.23M K2HPO4 aq./CH3OH=80/20

Flow rate : (Left); 0.8mL/min, (Center); 1.0mL/min, (Right); 0.8mL/min

Detector : (Left); UV(278nm), (Center); Shodex RI, (Right); UV(278nm)

Column temp.: (Left); 30°C, (Center); 50°C, (Right); 30°C