Guidelines for Shodex Column Selection : Proteins, Peptides and Amino Acids
Proteins and peptides are commonly studied in biotechnology, pharmaceuticals and food science. HPLC is one of the most common analytical methods for proteins or amino acids. The main separation modes used for protein analysis are gel filtration chromatography (size exclusion chromatography), reverse phase chromatography, ion exchange chromatography, hydrophobic interaction chromatography, affinity chromatography, etc. For analysis of peptides and amino acids, a combination of HILIC mode and LC/MS are also used.