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Adenosine and Derivatives

Adenosine and derivatives were analyzed using Asahipak GS-320 HQ (a multimode column).

Sample : 100μg/mL each, 20μL
1. ATP
2. ADP
3. AMP
4. Adenine
5. Adenosine



Column : Shodex Asahipak GS-320 HQ (7.5mmI.D. x 300mm)
Eluent : 200mM NaH2PO4 aq./ 200mM H3PO4 aq.=500/62
Flow rate : 1.0mL/min
Detector : UV(260nm)
Column temp. : 30°C