, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Comparison of Peak Height of GS-320 HQ with Semi-micro Columns

In the analysis of uracil with the conventional typed LC systems having injector, detector and tubes designed for the down sized analysis, conventional typed column GS-320 HQ was compared with its semi-micro typed columns. The peak height of uracil analyzed with semi-micro GS320A-2E (2.0mm I.D.) column is twelve times than that analyzed with conventional typed GS-320 HQ (7.5mm I.D.). And the peak height of uracil analyzed with semi-micro GS320A-1E (1.0mm I.D.) column is thirty six times than that analyzed with conventional typed GS-320 HQ (7.5mm I.D.). The high sensitivity can be obtained with your LC system and parts designed for down sized chromatography.

Sample : 10ppm each, 1μL
Uracil



Columns : Shodex GS320A-1E (1.0mmI.D. x 250mm) Shodex GS320A-2E (2.0mmI.D. x 250mm) Shodex Asahipak GS-320 HQ (7.5mmI.D. x 300mm)
Eluent : 10mM HCOONH4 buffer(pH4.0)
Detector : UV(260nm) cell volume; 3μL, optical path length; 7mm Tubing : 0.1mmI.D. PEEK tube Injector : Rheodyne 8125