, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Ribosomel RNA (2) (DEAE-420N)

Ribosomel RNA were analyzed using IEC DEAE-420N (a column for weak anion exchange chromatography packed with non-porous gel).
DEAE-420N is an old type of DEAE3N-4T.

Sample : 16s+23s-RNA (E.Coli MRE600)



Column : Shodex IEC DEAE-420N (4.6mmI.D. x 35mm)
Eluent : (A); 25mM Tris-HCl buffer(pH7.5) (B); (A) + 1M NaCl Linear gradient: 0min to 60min, 25% (B) to 100% (B)
Flow rate : 0.5mL/min
Detector : UV(260nm)
Column temp. : 30°C