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Comparison of Amino Column with Amide Column

It has been reported that the anomer separation does not take place under strong alkaline conditions. Amino columns such as Asahipak NH2P-50 4E allow the separation of saccharides without causing the anomer separation as they can be used under alkaline conditions at room temperature. (Please refer to .)
Amide columns are also used for the separation of saccharides. However, acryl-amide goups are not alkaline, it is necessary to perform the separation at elevated temperature to prevent anomer separation.



Sample : 5mg/mL each, 10μL
1. Fructose
2. Glucose
3. Sucrose
4. Maltose


 

 


Column : Shodex Asahipak NH2P-50 4E, Amide
Column from other manufacturer (4.6mmI.D. x 250mm each)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C