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Comparison of Columns with Different Counter Ions

Comparison of columns with different counter ions, Pb2+ (SP0810 ), Ca2+ (SC1011 ) and Na+ (KS-801 ) is shown. For the separation of monosaccharides(glucose and fructose), the separation by KS-801 is not sufficient because the separation mode of KS-801 is mainly GFC mode and their molecular weights are too close to be separated by GFC mode. When SP0810 and SC1011 are used, monosaccharides can be separated well because ligand exchange mode works for these columns.
For the separation of oligosaccharides(maltotriose and maltose), KS-801 is the best suited. The difference is caused by the difference of peak width. In case of SC1011 and SP0810, ligand exchange mode works and anomers are separated by the mode. And, as the consequence, the peak width become larger. Since the ligand exchange mode of SP0810 is stronger than that of SC1011, the separation of oligosaccharides by SP0810 is the worst.
As the conclusion, we can say that monosaccharides can be separated well by ligand exchange mode and oligosaccharides can be separated well by GFC mode.
Sugar alcohol(sorbitol) is separated by the ligand exchange mode. In case of SP0810, the elution of sorbitol is too slow because ligand exchange mode is working too strongly.

Sample :
1. Pullulan
2. Maltotriose
3. Maltose
4. Glucose
5. Sorbitol
6. Fructose
7. Glycerol
8. Ethanol



Columns : Shodex SUGAR SP0810, SC1011, KS-801 (8.0mmI.D. x 300mm each)
Eluent : H2O
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : 80°C