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Effects of Acetonitrile Concentration on Plate Number

When Asahipak NH2P-50 column is used, plate number of saccharides becomes higher as the acetonitrile concentration in the eluent becomes higher.
When silica-based amino columns are used, plate number and detection sensitivity of saccharides are different according to the type of saccharides, and, the plate number of some specific saccharide such as galactose is extremely low.
Please refer to .

Sample :
1. Rhamnose
2. Xylose
3. Fructose
4. Glucose
5. Sucrose
6. Maltose
7. Lactose
8. Galactose
9. Raffinose
10. Mannose


 


Columns : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm), Silica based amino
Column (other manufacturer)
Eluent : CH3CN/H2O
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C