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Durability Against Acidic Solvents

Since Asahipak NH2P-50 column is chemically stable, the durability against acidic solvents is better than that of silica-based amino columns.

Testing procedure

(1)Testing(63hrs)
Eluent : 0.01M H2SO4(pH2)
Flow rate : 0.1mL/min
Column temp. : Room temp.
(2)Replacement(1hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.
(3)Equilibrium(1hr)
Eluent : 0.1M NaOH
Flow rate : 0.5mL/min
Column temp. : Room temp.


Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)
Plate numberElution volume
(mL)
Plate number
1.Fructose 6.7396006.708400
2.Glucose 8.5979008.588500
3.Sucrose 11.88960011.939300
4.Maltose 14.24850013.908600

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C