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Durability Against Alkaline Solvents

Since the packing material of Asahipak NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.

Testing procedure

(1)Equilibrium(1hr)
Conditions before testing
Eluent : 100mM Ammonium acetate(pH9.3)
Flow rate : 0.1mL/min
Column temp. : Room temp.
(2)Analysis

(3)Testing(160hrs)
Eluent : 5mM NaOH(pH11.4)/CH3CN=25/75
Flow rate : 0.1mL/min
Column temp. : 30°C
(4)Replacement(0.5hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.
(5)Replacement(0.5hr)
Eluent : H2O
Flow rate : 0.5mL/min
Column temp. : Room temp.
(6)Analysis



Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)
Plate numberElution volume
(mL)
Plate number
1.Fructose 7.5271007.628100
2.Glucose 9.6361009.687700
3.Sucrose 13.41840013.409700
4.Maltose 16.16720016.087400


Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C