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Calibration Curves for Saccharides (NH2P-50)

Typical saccharides were analyzed using Asahipak NH2P-50 4E and calibaration curves for the saccharides were obtained. Every calibaration curves passes the origin and has good linearity. All the corelation coefficients for the saccharides are greater than 0.999. The calibration curves were obtained from peak heights. They can be also obtaind from the peak area satisfactorily.


Sample : 20μL

Correlation Coefficient
(0.2 to 200μg)
Glucose0.9998
Sorbitol0.9999
Fructose0.9998
Sucrose0.9999
Lactose0.9997
Maltose0.9998
meso-Erythritol0.9999
Xylitol0.9999
Mannitol0.9999
Maltitol0.9997



Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 25°C