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Comparison of NH2P with Silica-based Amino Column (2)

Compared with the case when Asahipak NH2P columns are used, when silica-based amino columns are used, detection sensitivity and plate number of saccharides are different according to the type of saccharides. And, the plate number of some specific saccharide such as galactose is extremely low.

Sample : 20μL
1. 2% Galactose
2. 1% Sucrose
3. 1% Maltose



Columns : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm), Silica based amino
Column (other manufacturer)
Eluent : CH3CN/H2O=75/25
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 25°C