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Mono-, Di- and Trisaccharides (1) (NH2P-50 4E)

Asahipak NH2P-50 is a
Column for the analysis of saccharides by normal phase mode. Under this mode, saccharides are basically eluted in increasing order of molecular weights. An increase of organic solvent in the
Eluent delays the elution of saccharides. NH2P
Column allows the separation of saccharides at room temperature without causing anomer separation as these
Columns are alkaline environment due to the bonded amino groups.

Sample : 0.5% each, 10µL
1. Rhamnose
2. Fructose
3. Glucose
4. Sucrose
5. Maltose
6. Raffinose

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)

Eluent : H2O/CH3CN=25/75

Flow rate : 1.0mL/min

Detector : Shodex RI

Column temp. : 30°C