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Monosaccharides and Disaccharides (3) (NH2P-50 4E)

In the Guidelines on Nutrition Labelling in Japan, amino
Columns of 4.6mm I.D. x 250 mm Length are adopted for the analysis of monosaccharides, disaccharides and sugar alcohols. This chromatogram shows the analysis result of typical monosaccharide and disaccharide standards using an amino
Column, Asahipak NH2P-50 4E. Since the
Column is polymer-based, it is durable compared with silica-based
Columns and can be washed by alkali solution.
The
Eluent used is the mixture of water and acetonitrile. The elution volume can be changed by changing the mixing ratio.

Sample : 0.1% each, 20μL
1. Fructose
2. Sorbose
3. Galactose
4. Glucose
5. Xylobiose
6. Sucrose
7. Lactose
8. Maltose

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)

Eluent : CH3CN/H2O=75/25

Flow rate : 1.0mL/min

Detector : Shodex RI

Column temp. : 30°C