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Yogurt: Sugar added (NH2P-50)

When analyzing saccharides in food, it is necessary to remove the substances besides saccharides before analysis. This procedure is called as sample pre-treatment. Here, the analysis result of saccharides in food using Asahipak NH2P-50 4E is shown together with the sample pre-treatment procedure.
(Sample pretreatment)
1) Take 5g of sugar added yogurt in a beaker, add 30mL of pure water and neutralize with 10%(w/v) NaOH.
2) Apply ultrasonic vibration for 30 minutes and make 50 mL solution.
3) Filtrate with No.5B paper filter and then with 0.45μm membrane filter.
The comparison of NH2P-50 column and silica-based amino column for the analysis of yogurt(sugar added) is as follows:
1)The peaks of fructose and galactose cannot be detected using silica-based amino column.
2)In case of NH2P-50 column, the peak of lactose is lager compared with the case of silica-based amino column. This result shows the fact that most of lactose is adsorbed by silica-based amino column. Since the adsorption of lactose by NH2P-50 column is not so much, the detection limit of NH2P-50 column is lower than that of silica-based amino column.

Sample : Yogurt , 20μL
1. Fructose
2. Galactose
3. Glucose
4. Sucrose
5. Lactose



Columns : Shodex Asahipak NH2P-50 4E, Amino
Column from other manufacturer (4.6mmI.D. x 250mm each)
Eluent : CH3CN/H2O=75/25
Flow rate : 0.6mL/min (NH2P-50), 1.0mL/min (Amino
Column from other manufacturer)
Detector : Shodex RI
Column temp. : 25°C