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Saccharides in powdered Milk

High sensivity analysis of saccharides in powdered milk was performed using Asahipak NH2P-50 4E (a column for saccharides analysis) and an electrochmical detection system.

Sample : powdered Milk
1. Lactulose
2. Lactose


Instrument        : Agilent 1050 system
Reagent           : 0.6M LiOH 0.8mL/min
Mode              : pulse
Working electrode : Gold
Potential         : Pot 1=0.65V, Pot 2=-0.95V, Pot=0.15V

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : (A); CH3CN/H2O=80/20 (B); H2O 0min to 40min, 5% (B) to 40% (B) 40min to 45min, 40% (B)
Flow rate : 1.0mL/min
Detector : Agilent 1049A Electrochemical
Column temp. : 40°C