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Effects of Organic Solvent in Eluent

Effects of organic solvent in eluent when saccharides are analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis) is shown.

Sample :
1. Fructose
2. Glucose
3. Sucrose
4. Lactose
5. Maltose



Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : (left); H2O/CH3CN=25/75 (right); H2O/C2H5OH=10/90
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : 30°C