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Comparison between NH2P-40 3E and its Conventional Type (NH2P-50 4E)

Asahipak NH2P-40 3E is filled with smaller-sized gel, 4μm, and packed in a 3.0 mm internal diameter (I.D.) column housing. The selected I.D. size allows the column to be used with a conventional LC system, i.e., without a need of semi-micro or any other special LC system. NH2P-40 3E provides higher sensitivity compared to Asahipak NH2P-50 4E.
Features of Shodex Asahipak NH2P-40 3E
1. Suitable for saccharides analysis by HILIC mode
2. Achieves 3 - 4 times improved detection sensitivity even with a conventional HPLC system
3. Uses less tha 50 % solvent compared to NH2P-50 4E


Sample : 0.2% each, 10μL
1. Arabinose
2. Mannose
3. Glucose
4. Sucrose
5. Lactose
Data obtained by a conventional RI (0.25 mm I.D. tubing).


Columns : Shodex Asahipak NH2P-40 3E (3.0mmI.D. x 250mm), NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : H2O/CH3CN=25/75
Flow rate : (NH2P-40 3E) 0.35mL/min, (NH2P-50 4E) 1.0mL/min
Detector : Shodex RI
Column temp. : 30°C