, , , , , , , , , , , , , , , , , , , , , , , , , , , , , , ,
+49 89 9399 6237 info(at)shodex.de Konrad-Zuse-Platz 3, München Mo-Do 8:30 - 17:00 / Fr 8:30 - 16:00
Konrad-Zuse-Platz 3, München   

Analysis of Trehalose According to JP Method (KS-801)

According to JP (Japanese Pharmacopoeia) method, assay of trehalose hydrate, it is necessary for the system suitability to satisfy the following.
- The elution order : maltotriose, trehalose, glucose and the internal standard (= glycerin)
- The resolution : ≥ 1.5 (maltotriose / trehalose), ≥ 4 (trehalose / glucose) and ≥ 3 (glucose / the internal standard)
- The relative standard deviation (RSD) of the ratio of the peak area of trehalose to that of the internal standard  : ≤ 1.0% (repeated six times)
It was confirmed that the all conditions were satisfied when analyzed using SUGAR KS-801, a
Column for sugar analysis.

Sample : 0.5% each, 20μL
1. Maltotriose
2. Trehalose
3. Glucose
4. Glycerin

Column : Shodex SUGAR KS-801 (8.0mmI.D. x 300mm)

Eluent : H2O

Flow rate : 0.4mL/min

Detector : Shodex RI

Column temp. : 80°C