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Hydrolyzed Dextran (3) (NH2P-50 4E)

Asahipak NH2P-50 4E is used for the separation of hydorolyzed dextran, and two methods, isocratic elution and gradient elution are compared. Using gradient elution, better separation can be obtained. Until recently, gradient elution could not be used for the separation of sugars because there was no
Detector to be used. ELSD (evaporative light scattering
Detector) solved this problem and enables the separtion of sugars using gradient elution.

Sample : 0.5% Hydrolyzed Dextran

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)

Eluent : (A); CH3CN/H2O=50/50 : (B); Linear gradient: 0min to 7 min, CH3CN/H2O=57/43 to 50/50 7min to 16 min, CH3CN/H2O=50/50

Flow rate : 1.0mL/min

Detector : ELS

Column temp. : Room temp.