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Phosphorylated Saccharides (2) (NH2P-50 4E)

Glucose can be anearobically decomposed into lactic acid and ethanol.This decomposing process is called as glucolytic pathway. In this pathway, some products such as glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, etc. are produced as intermediary products. Here, fructose-6-phosphate and fructose-1,6-bisphosphate are analyzed using Asahipak NH2P-50 4E by ion-exchange mode.

Sample : 5 mg/mL each, 20μL
1. Fructose-6-phosphate
2. Fructose-1,6-bisphosphate


Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : 300mM Sodium phosphate buffer(pH4.4)
Flow rate : 1.0mL/min
Detector : Shodex RI
Column temp. : 40°C