HILICpak VG-50 2D, a polymer based amino
Column, was used for LC/MS/MS analysis of saccharides and sugar alcohols. Adding ammonia to the
Eluent enhances the ionization of saccharides, and is effective for high sensitive detection.
Sample : 50ng/mL each (in H2O/CH3CN=1/9), 5μL meso-Erythritol Arabinose Xylose Fructose Mannose Glucose Sucrose Lactose Maltose
Column : Shodex HILICpak VG-50 2D (2.0mmI.D. x 150mm)
Eluent : (A); 0.1% NH3 aq. (B); CH3CN Linear gradient ; (B%) 95% (0 to 5min), 95% to 80% (5 to 15min), 80% (15 to 20min)