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Analysis of Mogroside V in Momordica Grosvenori (NH2P-50 4E)

Mogroside V is a natural sweetener that is included in Momordica Grosvenori, and it is said that the sweetness of Mogroside V is several hundred times of sucrose. Morgroside V in Momordica Grosvenori was analyzed by Asahipak NH2P-50 4E, a polymer-based amino column.
(Pretreatment)
1) Exactly weighting 0.2 g of Momordica Grsvenori, and exactly diluting to 10 ml total with the eluent.
2) Leaving it quietly for 2 hours.
3) Filtering it by a 0.45um-membrane-filter before injecting into HPLC.


Sample : 20μL
1. Mogroside V

Column : Shodex Asahipak NH2P-50 4E (4.6mmI.D. x 250mm)
Eluent : H2O/CH3CN=26/74
Flow rate : 1.0mL/min
Detector : UV(203nm)
Column temp. : 40°C