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Cleaning Method of Line Filter NH2P-LF

When Asahipak NH2P-50 is used, it is recommended to use NH2P-LF with it. Here, the way how to clean NH2P-LF and NH2P-50 is explained.
(1) When NH2P-LF is not used with NH2P-50, the column performance will deteriorate from just after it is used.
(2) When NH2P-LF is used with NH2P-50, the column performance is unchanged for considerably long time.
(3) Although NH2P-LF is used with NH2P-50, the column performance will deteriorate after it is used for a certain period. To prevent the deterioration, it is necessary to clean NH2P-LF at regular interavals.
(4) Before the deterioration of the column start, it is necessary to clean just NH2P-LF to maintain the column performance.
(5) After the deterioration of the column start, it is necessary to clean both NH2P-LF and NH2P-50 to regenerate the column performance.



(Cleaning method)
Dismount NH2P-LF and NH2P-50 from the HPLC system, and connect NH2P-LF or NH2P-50 to the pump in the reverse way. The flow rate should be 1.0mL/min for NH2P-LF and 0.5mL/min for NH2P-50. NH2P-LF and NH2P-50 should be cleaned separately according to the way described in the right table.
Do not connect anything after NH2P-LF or NH2P-50. If a detector is connected, detector cell may be damaged by the alkaline solvent. And if NH2P-50 is connected after NH2P-LF, the extraneous substances in the NH2P-LF may move to NH2P-50 and cause the deterioration of NH2P-50.

Cleaning
solvent
NH2P-LFNH2P-50 4DNH2P-50 4E
1. H2O
5 min
6 min
10 min
2. 0.1M HClO4
60 min
75 min
120 min
3. H2O
5 min
6 min
10 min
4. 0.1M NaOH
60 min
75 min
120 min
5. H2O
10 min
12 min
20 min
6. Eluent
30 min
40 min
60 min