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Analysis of Trehalose According to USP Method (KS-801)

According to USP 40 method, the assay of trehalose and the analysis of related substances in it should be analyzed using a
Column packed with L58. It is necessary for the system suitability to satisfy resolution (Rs) of trehalose and maltotriose of ≥ 1.5 and relative standard deviation (RSD) of peak area of trehalose ≤ 2.0%. It was confirmed that SUGAR KS-801 satisfies all conditions.

Sample : 0.25% each, 20µL
1. Maltotriose
2. Trehalose
3. Glucose

Column : Shodex SUGAR KS-801 (8.0mmI.D. x 300mm)

Eluent : H2O

Flow rate : 0.4mL/min

Detector : Shodex RI

Column temp. : 80℃