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Analysis of Ribose According to USP Method (KS-801)

According to USP 39 method, ribose should be analyzed using a
Column packed with L22. It is necessary for the system suitability to satisfy resolution of ribose/arabinose of ≥ 1.2, tailing factor of ≤ 1.5, theoretical plate number of ≥ 2,500 and relative standard deviation (RSD) of ≤ 2.0%. It was confirmed that the all conditions were satisfied when SUGAR KS-801 was used for analysis.

Sample : 10µL
1. Arabinose 0.02%
2. Ribose 2%

Column : Shodex SUGAR KS-801 (8.0mmI.D. x 300mm)

Eluent : H2O

Flow rate : 1.0mL/min

Detector : Shodex RI

Column temp. : 80°C