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Analysis of Mannitol according to EP Method (SC1011-7F)

According to EP method (Reference No. 0559, Version Date 04/2009), mannitol should be analyzed using a column which can separate mannitol and sorbitol with resolution of equal to or greater than 2.0. EP SC1011-7F is a column specially designed for mannitol analysis, whcih satisfies this criterion.

Sample : 5% each, 5μL
1. Mannitol
2. Sorbitol

Sample : 0.1% each, 20μL
Mannitol
Sorbitol
Maltitol
Isomalt (=Palatinit )



Column : Shodex EP SC1011-7F (7.8mmI.D. x 300mm)
Eluent : H2O
Flow rate : 0.5mL/min
Detector : Shodex RI
Column temp. : 85°C