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Absolute Molecular Weight Determination of Sodium Alginate (LB-806M)

Absolute molecular weight of sodium alginate was determined by OHpak LB-806M, a
Column for aqueous SEC. LB-800 series are compatible with multi angle light scattering (MALS)
Detector. By minimizing the baseline noise level, LB-800 series enable MALS
Detector to detect low molecular weight compounds which are difficult to be detected by conventional
Columns.

Sample : 100μL
0.1% Sodium alginate

Columns : Shodex OHpak LB-806M (8.0mmI.D. x 300mm) x 2

Eluent : 0.1M NaNO3 aq.

Flow rate : 1.0mL/min

Detector : MALS(Multi angle light scattering), Shodex RI

Column temp. : 30°C