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LC/MS Analysis of Various Steviol Glycosides (NH2P-40 2D)

Various steviol glycosides which are used as sweetener were analyzed using Asahipak NH2P-40 2D, a polymer-based amino
Column, with LC/MS/MS detection. Steviolbioside and Rebaudioside B which have carboxyl group can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.

Sample : 5μL
50ng/mL each
(in H2O/CH3CN=1/1)
Isosteviol
Stevioside
Rebaudioside C
Rebaudioside F
Rebaudioside A
Steviolbioside
Rebaudioside D
Rebaudioside B

Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)

Eluent : (A) 0.1% NH3 aq./(B) CH3CN Isocratic ; (B%) 78%

Flow rate : 0.2mL/min

Detector : ESI-MS (SIM Negative)

Column temp. : 30°C