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LC/MS Analysis of Various Sweeteners (NH2P-40 2D)

Various sweetners were analyzed using Asahipak NH2P-40 2D, a polymer-based amino
Column, with LC/MS/MS detection. Anion substances can be eluted under alkaline conditions. Furthermore, alkaline conditions promote the deprotonation of hydroxyl groups at the time of ionization. Therefore, alkaline conditions are suitable for high sensitive detection of substances with hydroxyl groups such as saccharides under the negative mode.

Sample : 5μL
50ng/mL each
(in H2O/CH3CN=1/3)
Sucralose
Acesulfame
Aspartame
Saccharin
Fructose
Glucose
Rebaudioside A
Sucrose
Lactose

Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm)

Eluent : (A) 0.1% NH3 aq./(B) CH3CN Isocratic ; (B%) 80%

Flow rate : 0.2mL/min

Detector : ESI-MS (SIM Negative)

Column temp. : 30°C