Sweeteners in an energy drink were analyzed using Asahipak NH2P-40 2D, a polymer-based amino
Column, with LC/MS/MS detection. Under these conditions, sweeteners, amino acids and water-soluble vitamins such as carnitine and pyridoxine can be simultaneously analyzed.

Sample : 5μL
500-fold diluted Energy drink with H₂O/CH₃CN=1/1
Sucralose
Acesulfame (k)
meso-Erythritol
Carnitine
Leucine
Isoleucine
Valine
Pyridoxine
Rebaudioside A

Column : Shodex Asahipak NH2P-40 2D (2.0mmI.D. x 150mm) 


Eluent : (A) 0.1% NH3 aq./(B) CH3CN  Isocratic ; (B%) 78% 


Flow rate : 0.2mL/min 


Detector : ESI-MS (SIM Positive/Negative) 


Column temp. : 30°C